DNA Fingerprinting Diagnostics (Replication, PCR and RFLP)

Kit #75

$291.95

DNA Fingerprinting Diagnostics (Replication, PCR and RFLP)

This timely Lab-Aid introduces biotechnology, forensic science, or AP Biology students to the concepts of DNA replication and how it relates to PCR (Polymerase Chain Reaction) and RFLP (Restriction Fragment Length Polymorphism) analyses of DNA. They also observe how DNA analyses can be used to diagnose disease. This activity is a great introduction or review if you are fortunate enough to have the equipment and materials to do gel electrophoresis and DNA fingerprinting. Material in this kit, for 30 students, is reusable.

Details at a Glance

1 Day | 1 ~50 minute class period
1 Activity
Accommodates 1 class, with 10 groups of 3 students
Consumables service 30 students
Meets our criteria for supporting literacy

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Scientific Concepts Guides & Worksheets Kit Components

Scientific Concepts

• Construct specific genes with the characteristic nucleotide configuration for cystic fibrosis
• Observe the difference between normal and mutant genes
• Understand how the disease can be diagnosed by two methods of DNA analysis
• Demonstrates amplification and PCR ( Polymerase Chain Reaction)
• Simulate gel electrophoresis

Guides & Student Sheets

Our kits and modules provide you with everything you need so you can open, review, and teach the material confidently the next day.

Full downloadable Teacher Guide with background information, detailed instruction, example data and answers
Downloadable Student Sheets with age appropriate background information, full procedure(s), and analysis questions
All materials necessary to carry out the investigation
Safety Data Sheets

Kit Components

1 Teacher’s Guide
20 Student Worksheets and Guides
10 Nucleotides sheet: cytosine 28 (blue)
10 Nucleotides sheet: guanine 28 (yellow)
10 Nucleotides sheet: adenine 66 (red)
10 Nucleotides sheet: thymine 66(green)
10 DNA stands, labels, sheets
2 Mutant CFTR
2 Normal CFTR
2 Primer 1
2 Primer 2
2 Template-Mutant CFTR
2 Template-Normal CFTR
10 Electrophoresis Bands, labels, sheets
1 Mutant CFTR PCR product (22 bp)
1 Mutant CFTR RFLP product (10 bp)
1 Mutant CFTR RFLP product (14 bp)
1 Normal CFTR PCR product (25 bp)
1 Normal CFTR RFLP product (25 bp)
60 Adhesive strips sized for 4 bp
30 Adhesive strips each, sized for 18, 21, 22, 25 bp
10 DNA Polymerase
3 Glue sticks, removable
10 Bags, resealable
10 Taql Restriction enzyme
10 Workboards, two-sided